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Mary was the only surviving child of george washington parke custis, george washington's stepgrandson, and mary lee fitzhugh custis, daughter of william fitzhugh [41] and ann bolling randolph. To examine the level of genetic differentiation between individuals we performed a principal component analysis (pca) of snp genotypes ( supplementary information ). Orbison, roy: laminar flow (asylum 6e 198 lp) 10 vgm- 1979,co.